Generation of Knock-In Syrian Hamsters via Zygote Microinjection Using CRISPR/Cas9 Genome Editing.

ABSTRACT: Syrian hamsters (Mesocricetus auratus) have long served as valuable model organisms in diverse research fields such as oncology, immunology, and physiology owing to their unique biological and pathological characteristics. Although embryo manipulation techniques such as embryo collection, pronuclear microinjection, and embryo transfer have been established, gene knock-in (KI) hamsters have not yet been reported. Here, we report the successful generation of gene KI Syrian hamsters by microinjecting CRISPR/Cas9 components and plasmid DNA into pronuclear-stage zygotes. Targeted insertion of a DNA cassette up to 8 kb was achieved at the ROSA26 orthologous locus and other genomic sites. Importantly, we confirmed functional expression of a reporter cassette inserted at the ROSA26 site, providing

SUBMITTER: Shigeta M 

PROVIDER: S-EPMC12444848 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature