Bioprospecting Heavy-Metal Rhizospheres for Novel Therapeutics Against High-Priority Multi-Drug-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii: A Case of Toxic to Treatment.

ABSTRACT: This study investigated the antimicrobial potential of rhizospheric microbiota isolated from heavy-metal-contaminated soils against two extremely drug-resistant (XDR) pathogens, Pseudomonas aeruginosa (strain ATCC 27853) and Acinetobacter baumannii (strain ATCC-BAA-1605). Heavy-metal-contaminated rhizospheres were sequentially exposed to ex-situ and in vitro enrichment with heavy metals from battery waste and incubated for 168 h. The surviving microbiota were screened against P. aeruginosa and A. baumannii, and crude extracts of high-performing strains were tested against the pathogens using agar well diffusion assays. The novelty and components of the extracted secondary metabolites from environmentally stressed rhizospheric microorganisms were inferred using ultra-performance liquid chro

SUBMITTER: Millard KB 

PROVIDER: S-EPMC12444940 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature