SwsB Acts as a Muramic-δ-lactam Cyclase in <i>Bacillus subtilis</i> Spore Peptidoglycan Synthesis.

ABSTRACT: The cortex layer of the peptidoglycan cell wall surrounding bacterial spores contains a modified sugar, muramic-δ-lactam, that is essential for spore germination. Genetic evidence has linked the conserved enzyme SwsB to the muramic-δ-lactam biosynthetic pathway. SwsB belongs to a large family of metal-dependent deacetylases, but its function is unclear because a putative catalytic residue is mutated. We have used native cortex peptidoglycan substrates to show that SwsB acts not as a deacetylase but as a monofunctional muramic-δ-lactam cyclase, the first enzyme reported with this activity. SwsB is remarkable in that it catalyzes lactam synthesis by direct intramolecular condensation of a carboxylate and primary amine with no apparent requirement for chemical energy input. SwsB will accept a

SUBMITTER: Hopkins ME 

PROVIDER: S-EPMC12444981 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature