Single-vesicle Tracking of α-Synuclein Oligomers Reveals Pore Formation by a Three-Stage Model.

ABSTRACT: Neurodegenerative disorders, such as Parkinson's disease (PD) pose significant health challenges. A major hallmark of PD is the aggregation of α-synuclein into toxic oligomers (αSO) and fibrils. While many efforts focus on slowing disease progression, the molecular origins and mechanisms of αSO toxicity remain poorly understood, particularly regarding its proposed link to membrane disruption. To address this, we have developed a single-vesicle analysis platform for direct, and real-time measurements of αSO and membrane interaction. This platform allows us to demonstrate real-time translocation of dyes through αSO pores with single-particle resolution and use single-channel electrical recordings to analyze pore formation in planar lipid bilayers. Across methods, our data provide evidence fo

SUBMITTER: Brochner BV 

PROVIDER: S-EPMC12444998 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature