Direct In-Bone Protein Digestion With Subsequent LC Separation and Trap Ion Mobility MS Detection of Released Peptides as an Effective Tool for the Proteomic Characterization of Bone Tissues.

ABSTRACT: Common pathological changes in bone tissues like osteomas or exostoses remain not fully understood at the molecular level due to the difficulties in analyzing bone tissues in which they occur. Therefore, new rapid and powerful techniques are needed that could become routine tools for such analysis. The primary aim of this study was to evaluate whether direct in-bone tryptic protein digestion followed by LC separation and trap ion mobility MS detection and identification of released peptides is able to identify sufficient numbers of proteins in above mentioned bone tissues. The second aim was to verify whether the mathematical analysis of the obtained MS data would have a potential to distinguish pathological and control healthy bone tissues. It turned out that this approach made possible t

SUBMITTER: Peterkova L 

PROVIDER: S-EPMC12445404 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature