Warm temperature modifies cell fates to reduce stomata production in Arabidopsis.

ABSTRACT: Stomatal abundance decrease in Arabidopsis triggered by warm temperature is attributed to PIF4-mediated repression of SPEECHLESS (SPCH) expression. We identified the unknown developmental and transcriptional basis of this adaptive response. We traced stomatal lineages in vivo using cell-identity marker lines and mutants, quantified epidermal traits, and conducted RNA sequencing under oscillating temperatures. Prolonged warm temperature or PIF4-overexpression altered cell fates, inducing diverted stomatal precursors (DPs) that lacked stomatal fate, contributing to stomata reduction. DPs originated from meristemoids that lost SPCH expression, lacked MUTE expression, and exited the cell cycle. Short warm-temperature pulses allowed later recovery of SPCH expression and did not induce DPs or st

SUBMITTER: Saiz-Perez J 

PROVIDER: S-EPMC12445817 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature