Correlation Between Chimeric Antigen Receptor T-Cell Pharmacokinetic Data Measured by Flow Cytometry and Quantitative Polymerase Chain Reaction.

ABSTRACT: Chimeric antigen receptor (CAR)-T cells have antitumor efficacy in hematological and solid malignancies. Unlike small molecules or antibodies, CAR-T cells have unique kinetic profiles (distribution, expansion, contraction, and persistence). To quantify these dynamics, flow cytometry and qPCR are commonly used, each with distinct advantages and limitations. We analyzed the correlation between flow cytometry and qPCR quantification of CAR-T cells in subjects from 4 phase 1 clinical studies (individually and combined). We also explored factors that affect calculations of CAR-T cells and CAR transgene copy number, how these affect pharmacokinetic (PK) parameters determination for clinical studies, and associations with pharmacodynamic factors such as cytokine levels. We demonstrate that CAR tr

SUBMITTER: Wang W 

PROVIDER: S-EPMC12445920 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature