Integrative analysis of single-cell and microarray data reveals SPI1-centered macrophage regulatory signatures in ulcerative colitis.

ABSTRACT:

Background

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) marked by persistent inflammation and ulceration of the colonic mucosal lining. Macrophages regulate intestinal inflammation through distinct polarization profiles. Emerging evidence indicates that the transcription factor SPI1 is a critical regulator of macrophage activity and contributes to both the initiation and progression of UC.

Methods

In this study, single-cell RNA sequencing (scRNA-seq) was conducted to profile the transcriptomic landscape of macrophages in the intestinal tissues of UC patients. A gene regulatory network (GRN) was constructed using pySCENIC, which identified SPI1 as a distinct transcriptional regulator involved in macrophage activation. To pinpoint key downstream target

SUBMITTER: Yu Y 

PROVIDER: S-EPMC12446007 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature