Single-cell and bulk transcriptomic analyses reveal PANoptosis-associated immune dysregulation of fibroblasts in periodontitis.

ABSTRACT:

Background

PANoptosis is a newly recognized form of programmed inflammatory cell death implicated in numerous inflammation-related diseases. However, its precise role and underlying mechanisms in periodontitis (PD) remain unclear.

Methods

We analyzed single-cell RNA sequencing (scRNA-seq) on gingival tissues from PD patients and healthy individuals to profile cellular composition and quantify cell-type distributions. Functional enrichment analyses were used to explore PANoptosis and related pathways, with five gene set scoring methods applied to quantify PANoptosis activity in human gingival fibroblasts (HGFs). The expression of PANoptosis-related markers was validated by immunofluorescence staining and qPCR in HGFs and gingival tissues from PD model mice. Based on PANoptosi

SUBMITTER: Wu E 

PROVIDER: S-EPMC12446042 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature