Effects of a novel Paraburkholderia phage IPK on the phenanthrene degradation efficiency of the PAH-degrading strain Paraburkholderia caledonica Bk.

ABSTRACT: Phages are a major cause of bacterial mortality, affecting bacterial diversity and ecosystem functioning. However, the impact of phage-host interactions in contaminated environments and their role in pollutant biodegradation have largely been overlooked. We isolated and characterized a novel phage that infects the PAH-degrading bacterium Paraburkholderia caledonica Bk from a polycyclic aromatic hydrocarbon (PAH)-contaminated soil and investigated the effect of different multiplicity of infection (MOI) ratios on the degradation efficiency of phenanthrene. The phage IPK is a temperate phage with a wide pH and temperature tolerance and a burst size of 80  PFU ml⁻¹. The phage was classified as a member of the Caudoviricetes and is related to Pseudomonas and Burkholderia phages. Howe

SUBMITTER: Nieto EE 

PROVIDER: S-EPMC12446147 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature