MiR-26b-5p mediates radioresistance and immunosuppression via targeting PRKCD in non-small cell lung cancer.

ABSTRACT:

Purpose

Overcoming miRNA-mediated radioresistance and enhancing its synergy with immunotherapy remained significant challenges.

Methods

A total of 23 patients with locally advanced non-small cell lung cancer (NSCLC) undergoing thoracic radiotherapy from a single center were enrolled. Pre-radiotherapy blood samples were collected and analyzed using real time qPCR array to detect miRNA expression profiles, identifying differential miRNAs between responders and non-responders. In vitro experiments further assessed the impact of radiotherapy on significant differential miRNAs. Targeted immune genes of miRNAs were predicted through bioinformatics websites and validated by cellular experiments. Using TCGA and GEO datasets, the association between immune gene of interest and surviv

SUBMITTER: Chen X 

PROVIDER: S-EPMC12446157 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature