A human H5N1 influenza virus expressing bioluminescence for evaluating viral infection and identifying therapeutic interventions.

ABSTRACT: A multistate outbreak of highly pathogenic avian influenza virus (HPAIV) H5N1 in the United States dairy cattle was first reported on March 2024, followed by a zoonotic cattle-to-human virus transmission to a dairy farm worker in Texas. To facilitate real-time tracking of HPAIV H5N1, we generated a recombinant nanoluciferase (Nluc)-expressing H5N1 virus, HPhTX NSs-Nluc, by introducing an Nluc reporter into the non-structural gene of human A/Texas/37/2024 H5N1 (HPhTX). HPhTX NSs-Nluc replicated with kinetics and plaque morphology comparable to wild-type virus in vitro. In vivo and ex vivo, HPhTX NSs-Nluc allowed tracking viral infection in the living animals and their necropsied organs using in vivo imaging systems (IVISs). Treatment with baloxavir effectively in

SUBMITTER: Barre RS 

PROVIDER: S-EPMC12446200 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature