Durable tissue-specific transgene expression in newborn mice following intraperitoneal delivery of non-cytotoxic HSV vectors.

ABSTRACT: This report describes the distribution and transgene expression of two non-cytotoxic, replication-defective (rd) herpes simplex virus (HSV) vectors, JΔNI7 and JΔNI8, following intraperitoneal delivery to newborn mice. The two vectors are functionally defective for all immediate-early genes, and JΔNI8 is further deleted for the UL41 endonuclease (vhs). Both vectors were engineered to express a red luciferase gene from the LAT locus to track vector distribution and gene expression in vivo. A comparison of reporter gene activities under the control of four different promoters in JΔNI7 showed that the strongest expression was achieved with the CAG promoter. Distribution analysis at 1 week post-injection showed transgene expression in multiple tissues, but at 4 weeks, high-level e

SUBMITTER: Miyagawa Y 

PROVIDER: S-EPMC12446203 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature