New diagnostic methods for <i>Escherichia marmotae</i> and the first report of its identification in clinical isolates in North America.

ABSTRACT:

Background

Genomic sequences of E. marmotae and E. coli differ by 10%. Discovered as an environmental "cryptic clade" of Escherichia, E. marmotae also occurs in human infections. Microbiological and MALDI-TOF-MS methods frequently misidentify E. marmotae as E.coli. Our goal was to develop methods that reliably distinguish E. marmotae from E. coli to improve therapeutic decisions and treatments.

Methods

A Taqman PCR method was developed to distinguish E. marmotae from E. coli based on genomic sequences of uidA, uidB, and a positive control targeting adk in E. marmotae and E. coli. MALDI-TOF-MS spectra were obtained for environmental and clinical isolates using a bioMérieux VITEK MALDI-TOF-MS system.<

SUBMITTER: Oladipo PM 

PROVIDER: S-EPMC12446263 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature