Eco-friendly spectrofluorimetric and HPLC-fluorescence methods for simultaneous determination of melatonin and zolpidem in pharmaceuticals.

ABSTRACT: Two eco-friendly, facile, rapid, and highly sensitive analytical methodologies were developed for the concurrent estimation of melatonin and zolpidem tartrate in raw materials and dosage forms. The first approach is a green first derivative synchronous spectrofluorimetric method (Method I), utilizing a wavelength interval (Δλ) of 60 nm and capturing derivative signals at 265.0 nm and 339.0 nm for melatonin and zolpidem, respectively. Method I exhibited excellent linearity over the ranges of 8.0-70.0 ng/mL for melatonin and 10.0-80.0 ng/mL for zolpidem, with detection limits of 1.62 ng/mL and 1.19 ng/mL. The second method (Method II) involves high-performance liquid chromatography coupled with fluorescence detection (HPLC-FD), employing a C18 column and a mobile phase of methanol:0.05% trie

SUBMITTER: Abo Elkheir SM 

PROVIDER: S-EPMC12446487 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature