Demineralized Dentin Matrix Promotes Bone Regeneration Through IDO1-Mediated Th17/Treg Cell Balance Modulation.

ABSTRACT:

Objectives

Demineralised dentin matrix (DDM) is an effective scaffold material for bone tissue engineering. However, the osteoimmunological mechanism of DDM remains unexplored. Th17/Treg cell balance has been noticed as a crucial factor in bone regeneration. The aim of this research is to elucidate the influence of DDM on the balance of Th17/Treg and its underlying molecular mechanisms.

Methods

The study examined DDM's effects on Th17/Treg cell balance by co-incubating DDM extract with peripheral blood mononuclear cells (PBMCs). Transcriptomics and bioinformatics identified DDM-related differentially expressed genes. A naïve CD4⁺ T cell-bone marrow-derived mesenchymal stem cells (BMSCs) co-culture model assessed DDM's immunomodulatory effects on Th17/Treg differentiation and

SUBMITTER: Zhu S 

PROVIDER: S-EPMC12446538 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature