Perinatal Maturation of Drug Transporters and Claudin-5 at the Blood-Brain Barrier.

ABSTRACT:

Aim

Cerebral capillary endothelial cells (EC) form the blood-brain barrier (BBB), which regulates molecular exchange between the blood and the brain. Understanding their function during brain development is essential for optimizing treatments in neonates, children, as well as pregnant and breastfeeding women.

Methods

P-glycoprotein (P-gp/ABCB1) expression during brain development was assessed by immunohistochemistry in human cortical samples. In mice, postnatal brain microvessels were analyzed using qPCR and Western Blot, and BBB function was evaluated in vivo using [¹⁴C]sucrose to assess barrier integrity, and [³H]verapamil or [³H]rosuvastatin to assess transport activity.

Results

In humans, P-gp reached mature levels in the early pos

SUBMITTER: Federici L 

PROVIDER: S-EPMC12446565 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature