Design, development, and testing of a new multi-locus sequence typing scheme for the zoonotic pathogen <i>Cryptosporidium parvum</i>.

ABSTRACT: The zoonotic parasite Cryptosporidium parvum is an important global cause of diarrheal disease in humans and young ruminants. Molecular typing is essential to track transmission routes and identify clusters of cases. Here, we developed a novel multi-locus sequence typing (MLST) scheme based on single nucleotide polymorphisms (SNPs) in unlinked markers. Coding regions with high variability were identified by comparing whole genome sequences (WGS) from 43 human- and 92 ruminant-derived C. parvum samples collected across Europe. We first selected 18 markers and showed that they provide high discrimination among the samples with WGS data, with 88% of the MLSTs being singletons. Next, we defined a MLST based on eight genetically unlinked markers and generated sequence data from 30

SUBMITTER: Troell K 

PROVIDER: S-EPMC12446620 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature