Small molecule agonist TPC2-A1-N increases intracellular Ca²⁺ independent of two-pore channels.

ABSTRACT: Two-pore channels (TPCs) constitute a small family of cation channels expressed in endo-lysosomal compartments. TPCs have been characterized as important constituents controlling Ca²⁺-mediated vesicular membrane fusion and fission, thereby regulating intracellular organelle trafficking. Two activators, nicotinic acid adenine dinucleotide phosphate and phosphatidylinositol-3,5-bisphosphate, induce ion flux through TPCs. The membrane-permeable small molecule activators TPC2-A1-N and TPC2-A1-P have been identified and postulated to mimic their action and to discriminate for a preferential selectivity either for Ca²⁺ or for Na⁺. This was observed only for TPC2 and was independent of nicotinic acid adenine dinucleotide phosphate-binding proteins. Here, we applie

SUBMITTER: Mallmann RT 

PROVIDER: S-EPMC12446622 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature