Obesity-Associated Adiposomes Promote Vascular Smooth Muscle Cell Hypercontractility.

ABSTRACT:

Background

Inter-organ crosstalk, particularly between adipose tissue and vasculature, plays a key role in obesity-induced cardiovascular dysfunction. Our previous work showed that adipose-derived extracellular vesicles (adiposomes) from obese donors impair arteriolar vasodilation through endothelial dysfunction, but their impact on vascular smooth muscle cell (VSMC) function remains unclear.

Methods

Visceral adipose tissues were collected from 25 obese and 12 lean subjects undergoing bariatric and elective surgeries, and from high-fat diet-induced obesity (DIO) mice (n = 40). Adiposomes were isolated by ultracentrifugation, and arteriolar myogenic tone was assessed using pressure myography. Intracellular Ca²⁺, membrane potential, and reactive oxygen species (ROS)

SUBMITTER: Metwally E 

PROVIDER: S-EPMC12446896 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature