Single-Nucleus RNA-Sequencing Identifies a Differential Profibrotic Response in Parietal Epithelial Cells in Primary Versus Maladaptive Focal Segmental Glomerulosclerosis.

ABSTRACT:

Introduction

Focal segmental glomerulosclerosis (FSGS) lesions occur in a wide range of clinical conditions that are all characterized by critical podocyte injury. Differentiating primary from maladaptive forms of FSGS remains challenging because of the absence of reliable biomarkers, resulting from a lack of insight into their pathophysiological differences.

Methods

We used single-nucleus RNA-sequencing (snRNA-seq) to identify differentially expressed transcriptional signatures in kidney biopsies of well-phenotyped primary versus maladaptive FSGS. We included cryopreserved kidney biopsy cores from adult patients with newly diagnosed primary FSGS (n = 9, all nephrotic), maladaptive FSGS (n = 9, all nonnephrotic), proteinuric controls (antiphospholipase A2 recep

SUBMITTER: Deleersnijder D 

PROVIDER: S-EPMC12446951 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature