MoMad2 With a Conserved Function in the Spindle Assembly Checkpoint Is Required for Maintaining Appressorial Turgor Pressure and Pathogenicity of Rice Blast Fungus.

ABSTRACT: Mad2, a conserved core component of the spindle assembly checkpoint (SAC) in eukaryotes, delays anaphase onset in case of incorrect kinetochore-microtubule attachment. However, its functions in plant-pathogenic fungi remain largely unknown. Here, we identified the Mad2 homologue in rice blast fungus Magnaporthe oryzae (MoMad2), which shows high similarity with Mad2 in fission yeast. When expressed in fission yeast, MoMad2 associated with native SpMad1 and SpCdc20, and successfully rescued the ΔSpmad2 mutant's defect in arresting anaphase onset upon damaged spindle, indicating the conserved SAC function of MoMad2. Moreover, MoMad2 interacted with MoMad1 and depends on MoMad1 for its nuclear envelope-localisation. Although it plays a dispensable role in M. oryzae growth, MoMad2 is required f

SUBMITTER: Shen T 

PROVIDER: S-EPMC12446995 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature