Unilateral isometric contraction induces REDD1 and suppresses insulin-stimulated mTORC1 and protein synthesis in non-contracted muscle of male mice.

ABSTRACT: Skeletal muscle hypertrophy is promoted by mechanical loading and is associated with activation of mTORC1 signaling. While REDD1, a stress-responsive inhibitor of mTORC1, is typically downregulated in contracting muscle, we previously reported that unilateral isometric contraction increases REDD1 expression in the contralateral non-contracted muscle. The functional significance of this response remains unclear. This study tested whether REDD1 induction in non-contracted muscle attenuates anabolic signaling under insulin-stimulated conditions. Male C57BL/6J mice underwent unilateral isometric contraction of the right gastrocnemius via percutaneous electrical stimulation. Insulin was administered systemically to stimulate mTORC1 signaling and protein synthesis. Western blotting was used to a

SUBMITTER: Amar MM 

PROVIDER: S-EPMC12446998 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature