<i>Aurantii fructus immaturus</i> (Rutaceae) flavonoid ameliorated constipation by regulating colonic microbiota and miRNA/mRNA network.

ABSTRACT: Aurantii fructus immaturus flavonoid (AFIF) is the main constituent of Aurantii fructus immaturus (Rutaceae) (AFI), and is effective against constipation. This study explores the mechanism of AFIF against antibiotics-induced constipation (AC) in mice. Forty six-week-old female C57BL/6 mice were randomly divided into 4 groups (n = 10): control, control + AFIF, model, and model + AFIF groups. The AC model was established by antibiotics mixture for 8 days. Mice were gavaged daily with AFIF (0.1 mL/10 g, 3 g/mL) for 2 weeks. Hematoxylin and eosin (H&E) staining and periodic acid-Schiff (PAS) staining were used for histological analysis. The colonic microbiota was analyzed by 16sRNA sequencing. Transcriptome sequencing was used to detect miRNA and mRNA expression profiles.

SUBMITTER: Wen Y 

PROVIDER: S-EPMC12447141 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature