DNA Hydrogel-Interfaced Organic Electrochemical Transistor for the Investigation of Binding-Induced Conformational Change of Small Molecule Aptamers.

ABSTRACT: Investigation of the small molecule-aptamer interaction is difficult, and it usually lacks information about the conformational change of aptamers that is important for their application. Here, we present the label-free investigation of small molecule-aptamer interactions using a modularized organic electrochemical transistor (OECT) platform. Leveraging the high sensitivity of the OECT, we measured the conformational change of the aptamer encountering its ligand. This platform consists of a simply fabricated OECT device and a DNA hydrogel-based bioreceptor module. Using this device, we investigated four DNA aptamers binding to cortisol (362.46 Da) and testosterone (288.4 Da). We demonstrated the correlation between the aptamer conformational change and the OECT response. We further calcula

SUBMITTER: Lin H 

PROVIDER: S-EPMC12447391 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature