In vivo tracking of transfused platelets by next-generation sequencing of mitochondrial DNA.

ABSTRACT:

Abstract

Tracking transfused platelets is important to evaluate platelet transfusion efficiency. Traditionally, corrected count increments were used; however, quantitative polymerase chain reaction-based methods have recently been developed. As both these methods have some limitations, we developed a new method based on next-generation sequencing (NGS) of platelet mitochondrial DNA (mtDNA). We identified several single nucleotide variant markers by sequencing the entire mtDNA region of platelets, and used NGS to estimate the proportion of each platelet unit. This method was validated using mixed platelets obtained from different donors at various ratios. We confirmed the applicability of this method in patients who received platelet transfusions using pre- and posttransfusion sampl

SUBMITTER: Choi SJ 

PROVIDER: S-EPMC12447411 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature