Comparison of PCR-Based Methods for the Detection of Canned Tuna Species.

ABSTRACT: Canned tuna is susceptible to mislabeling due to its high consumer demand, complex global supply chains, and range of prices. DNA barcoding of a short fragment of the mitochondrial control region (CR), termed the CR mini-barcode, has been established as an effective method for tuna species identification. However, the high level of DNA degradation in canned tuna products reduces the effectiveness of this method. Therefore, this study aimed to compare CR mini-barcoding to targeted (i.e., real-time or multiplex) PCR-based methods to determine the most effective approach for canned tuna species identification. DNA was extracted in duplicate from 24 canned tuna products labeled as albacore, skipjack, yellowfin, and light tuna. Each sample was analyzed with CR mini-barcoding, real-time PCR, and

SUBMITTER: Castanon CP 

PROVIDER: S-EPMC12447540 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature