NDUFS8 facilitates hepatocellular carcinoma growth by enhancing mitochondrial function and escaping HUWE1-dependent degradation.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide. Although mitochondrial metabolism contributes to tumorigenesis, the specific roles of individual mitochondrial components remain unclear.NADH:ubiquinone oxidoreductase core subunit S8 (NDUFS8), a key subunit of mitochondrial complex I, has been implicated in non-hepatic malignancies, but its functional relevance in HCC is unknown.

Methods

We assessed NDUFS8 expression in HCC tissues and cell lines using TCGA datasets and patient specimens. Functional analyses-including mitochondrial assays, apoptosis, proliferation, and migration-were performed in NDUFS8-silenced, knockout, and overexpressing HCC cells. In vivo tumor growth was evaluated using xenograft mouse models. Mechanist

SUBMITTER: Zhu X 

PROVIDER: S-EPMC12447896 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature