Thiol-ene click reaction aptamer sensor based on MWCNT-COOH/MOF-818 composite for highly sensitive detection of foodborne pathogenic bacteria.

ABSTRACT: This study established a unified electrochemical sensing platform for the ultra-sensitive and rapid detection of foodborne pathogens. Targeting three representative pathogenic bacteria-Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium-a composite sensing interface was fabricated by integrating MWCNT-COOH with MOF-818. Highly specific aptamers were directionally immobilized onto the MWCNT-COOH/MOF-818 composite surface through thiol-ene click chemistry, yielding an electrochemical biosensor with superior performance. Experimental results revealed detection limits of 3, 6, and 4 CFU mL^-1 for the three target bacteria, respectively. Moreover, the sensor displayed strong detection capabilities in authentic food samples (pork paste). This detect

SUBMITTER: Chi H 

PROVIDER: S-EPMC12447916 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature