PROS1 released by lung basal cells limits inflammation in epithelial and monocytes during SARS-CoV-2 infection.

ABSTRACT:

Introduction

Factors regulating the severity of pneumonitis during viral infections remain unresolved. We previously found higher expression of protein S (PROS1) in lung epithelium of mild compared to severe coronavirus disease 2019 (COVID-19) patients. We hypothesized that PROS1 may protect the upper airways by regulating epithelial and myeloid cell responses during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

Methods

To test this, in vitro air-liquid interface (ALI) cultures of primary healthy human lung epithelial cells were infected with SARS-CoV-2. This model, validated through immunofluorescent staining, confocal microscopy, and single-cell RNA-sequencing, replicated pathogenic changes seen in the lungs of COVID-19. Regulation and secr

SUBMITTER: Simakou T 

PROVIDER: S-EPMC12448451 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature