Dynamic sperm proteome remodeling during epididymal maturation in a marsupial, Macropus giganteus†.

ABSTRACT: While assisted reproductive technologies (ARTs) are widely used in domestic animals, successful implementation of ARTs to conserve wildlife species remains challenging. In macropods, crucial aspects of fundamental reproductive biology, including changes induced by epididymal maturation, remain unknown, limiting the development of ARTs. In this context, we performed a proteomic analysis of spermatozoa from the caput, corpus, and cauda epididymis of Eastern Grey Kangaroos (n = 6) to profile changes over epididymal maturation. Samples prepared by filter-aided sample preparation digestion were analyzed by liquid chromatography tandem mass spectrometry with sequential window acquisition of all theoretical acquisition. A total of 4304 proteins were identified, with significant overlap across epi

SUBMITTER: Kunkel S 

PROVIDER: S-EPMC12448624 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature