The role of auxin-mediated gene activation in the bryophyte, Physcomitrium patens.

ABSTRACT: Perception and response to the hormone auxin are critical to plant growth and development. Expression of auxin-response genes is tightly regulated via known mechanisms of both activation and repression. Across the plant lineage, auxin-response gene induction is performed by AUXIN-REPSONSE FACTOR (ARF) activating transcription factors. Conversely, AUXIN/INDOLE ACETIC ACID proteins repress expression. Studies of gain-of-function constitutive repression lines and ARF loss-of-function mutants have advanced the field. Yet, there is a need for a comparative study of aberrant auxin signaling mutants to understand the developmental consequences of constitutive repression versus the absence of auxin-mediated gene induction. Using CRISPR/Cas9 gene-editing tools, we mutated each activating ARF gene i

SUBMITTER: Bascom C 

PROVIDER: S-EPMC12448817 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature