Spatially resolved transcriptomics of benign and malignant peripheral nerve sheath tumors.

ABSTRACT:

Background

Peripheral nerve sheath tumors (PNSTs) encompass entities with different cellular differentiation and degrees of malignancy. Spatial heterogeneity complicates the diagnosis and grading of PNSTs in some cases. In malignant PNST (MPNST) for example, single-cell sequencing data has shown dissimilar differentiation states of tumor cells. Here, we aimed to determine the spatial and biological heterogeneity of PNSTs.

Methods

We performed spatial transcriptomics on formalin-fixed paraffin-embedded diseased peripheral nerve tissue. We used spatial clustering and weighted correlation network analysis to construct niche-similarity networks and gene expression modules. We determined differential expression in primary pathologies, analyzed pathways to investigate the biologic

SUBMITTER: Bremer J 

PROVIDER: S-EPMC12448819 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature