Translesion polymerases and DNA-protein crosslink repair shapes the cellular response to formaldehyde-induced DNA damage in ssDNA.

ABSTRACT: Formaldehyde (FA) is a highly reactive aldehyde that forms a variety of adducts with biomolecules, including DNA base adducts, interstrand crosslinks, intrastrand crosslinks, and DNA-protein crosslinks (DPCs). FA is produced from several exogenous and endogenous sources and has been linked to many cancer types. Persistent adducts are often bypassed by error-prone translesion synthesis (TLS) polymerases, causing mutations that contribute to the FA-induced mutational spectrum. However, it is unknown how DPC repair and TLS coordinate to bypass FA damage, and how bypass by different TLS polymerases alters FA's mutational spectrum. Here, we use an established yeast mutational reporter system to confirm that FA preferentially mutagenizes guanine residues within single-stranded DNA (ssDNA). We fi

SUBMITTER: Blouin T 

PROVIDER: S-EPMC12448846 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature