In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.

ABSTRACT: The budding yeast Saccharomyces cerevisiae plays an integral role in the bioeconomy as a powerful host for industrial bio-manufacturing, driving the production of diverse bio-based products. Achieving optimal product yields requires precise fine-tuning of the expression levels of multiple pathway genes, which often relies on cloning-intensive methods. Here, we present PULSE, an in vivo promoter engineering tool based on a streamlined workflow combining FACS-based screening of a randomized DNA library to identify active promoter elements, and their subsequent assembly into synthetic hybrid promoters where each element is flanked by loxPsym sites. Multiple promoter cassettes can be genome-integrated to generate "ready-to-use" platform strains, allowing users to easily place their genes of in

SUBMITTER: Ruehmkorff C 

PROVIDER: S-EPMC12448870 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature