First chromosome-level genome assembly of the colonial chordate model Botryllus schlosseri (Tunicata).

ABSTRACT:

Background

Botryllus schlosseri (Tunicata) is a colonial, laboratory model tunicate recognized for its remarkable developmental diversity, its regenerative abilities, and its peculiar genetically determined allorecognition system governed by a polymorphic locus controlling chimerism and cell parasitism.

Results

We report the first chromosome-level genome assembly of B. schlosseri subclade A1. By integrating long and short reads with Hi-C scaffolding, we produced both a phased diploid genome assembly and a conventional collapsed consensus sequence of 533 Mb. Of this total length, 96% belonged to 16 chromosome-scale scaffolds, with a BUSCO completeness score of 91.4%. We then compared our assembly with other high-quality tunicate genomes, revealing some synteny conservation bu

SUBMITTER: De Thier O 

PROVIDER: S-EPMC12448946 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature