Acute radiation exposure in mice reveals traces of oxidation-mediated modifications in serum albumin.

ABSTRACT: High doses of ionizing radiation induce specific oxidation-mediated modifications on the sequence of amino acid that constitutes serum albumin, but the time-dependent response thereafter is unclear. We, therefore, used liquid chromatography coupled with tandem mass spectrometry to profile oxidation-mediated modifications in serum collected from mice subjected to total body X-ray irradiation (TBI) at 20-day intervals up to day 80. In TBI mice, albumin structural regions were generally glycated and oxidatively modified, and in particular, oxidative modifications of serum albumin (OMSA) were observed on either analysis day in eight sequences containing the following amino acid residues: OMSA9 (oxidation of lysine residue (Lys)-97), OMSA12 (oxidation of methionine residue (Met)-147), OMSA21 (o

SUBMITTER: Yamaguchi M 

PROVIDER: S-EPMC12448996 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature