Charting the equine miRNA landscape: An integrated pipeline and browser for annotating, quantifying, and visualizing expression.

ABSTRACT: MicroRNAs (miRNAs) are essential regulators of gene expression, yet few comprehensive databases exist for miRNA expression in non-model species, limiting our ability to characterize their roles in gene regulation, development, and disease. Similarly, isomiRs - length and sequence isoforms of canonical miRNAs with potentially altered regulatory targets and functions - have received even less attention in non-model species, including the horse, leaving a critical gap in our understanding of their biological significance. To address these challenges, we developed an open-source, containerized pipeline for identifying and quantifying miRNAs and isomiRs (FARmiR: Framework for Analysis and Refinement of miRNAs), and an associated interactive browser (AIMEE: Animal IsomiR and MiRNA Expression Exp

SUBMITTER: Cullen JN 

PROVIDER: S-EPMC12449019 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature