IFN-β therapy rescues dysregulated IFN-stimulated proteins, serum cytokines, and neurotrophic factors in multiple sclerosis: Multiplex analysis of short-term and long-term IFN responses.

ABSTRACT:

Introduction

Dysfunctional regulatory T cells and subnormal responses to interferon-β disrupt the immune system in multiple sclerosis. We probed dysregulated type I IFN pathways in vitro and in vivo IFN-β to induce transcription factors, cytokines, and neurotrophic factors.

Methods

36 MS-relevant serum proteins curated to be relevant to MS were detected with multiplex and IFN activity assays plus western blots of mononuclear cells from 15 partial responders (PR) to IFN-β therapy with exacerbations over five years of treatment, and 12 clinical responders (CR) without exacerbations. Response was measured 0, 4, and 24 hours after injection of 16 million units (MU) of IFN-β (double-dose) and 8 MU (standard dose), in clinically stable PR and CR, and 16 MU IFN-β in paired PR durin

SUBMITTER: Li L 

PROVIDER: S-EPMC12449033 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature