PyVADesign: a python-based cloning tool for one-step generation of large mutant libraries.

ABSTRACT:

Motivation

The generation and analysis of diverse mutants of a protein is a powerful tool for understanding protein function. However, generating such mutants can be time-consuming, while the commercial option of buying a series of mutant plasmids can be expensive. In contrast, the insertion of a synthesized double-stranded DNA (dsDNA) fragment into a plasmid is a fast and low-cost method to generate a large library of mutants with one or more point mutations, insertions, or deletions.

Results

To aid in the design of these DNA fragments, we have developed PyVADesign: a Python package that makes the design and ordering of dsDNA fragments straightforward and cost-effective. In PyVADesign, the mutations of interest are clustered in different cloning groups for efficient exchang

SUBMITTER: Kuin RCM 

PROVIDER: S-EPMC12449254 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature