Systematic engineering to enhance valencene production in Rhodobacter sphaeroides.

ABSTRACT: Microbial synthesis of (+)-valencene from agricultural wastes such as cornstalk will serve as a sustainable alternative to the traditional plant extraction method. In this study, Rhodobacter sphaeroides strains were engineered for valencene production through a stepwise manner: (1) heterologous expression of Callitropsis nootkatensis valencene synthase (CnVS) in combination with phaB/gdhA/ladH knockouts enabled de novo biosynthesis of valencene from glucose at a titer of 34.21 ± 3.1 mg/L; (2) a quorum-sensing promoter P_cer to decouple growth and production phase further improved the valencene titer to 80.75 ± 3.0 mg/L; and (3) transposon-mediated genomic integration of the heterologous mevalonate pathway to enhance farnesyl pyrophosphate supply resulted in 120.53 ± 10.34 mg/L va

SUBMITTER: Li Z 

PROVIDER: S-EPMC12449282 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature