Flow Rate Determination of the Nanoflow Sheath Liquid CE-MS-Coupling Applying the nanoCEasy Interface.

ABSTRACT: In recent years, nanoflow sheath liquid (nanoSL) interfaces have been used more commonly for capillary electrophoresis-mass spectrometry (CE-MS) coupling due to their high sensitivity combined with flexibility in CE separation conditions. So far, the exact amount of sheath liquid (SL) and, thus, the dilution effect remain unknown due to the self-supplying type of the nanoSL interfaces. To quantify the SL flow rates, we present here an approach for the determination of the flow rate based on isotopically labeled standards using the nanoCEasy interface. SL and pumped CE flow are spiked with atrazine and d5-atrazine, respectively, enabling the determination of the total flow rate for different electrospray (ES) conditions. Generally, flow rates increase with higher ES voltages. Only at high E

SUBMITTER: Hochsmann A 

PROVIDER: S-EPMC12449746 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature