Optical Determination of the Internal Capillary Diameter Used in Taylor Dispersion Analysis.

ABSTRACT: In this work, we describe an optical setup to determine the internal diameter of narrow bore fused silica capillary used in capillary electrophoresis and Taylor dispersion analysis (TDA). Indeed, fluctuations up to about ±3-4 µm on the capillary I.D. can generate important inaccuracy on the hydrodynamic radius determination by TDA. Calibration of the optical set-up, impact of the operator and of the placement of the capillary in the focal plane, and the influence of the way to cut the capillary were investigated and discussed. This optical set-up was next used to determine capillary I.D. on a 60 m long capillary spool. Relatively small variations were observed along a 60 m capillary spool (0.3 µm maximum variation), while important I.D. fluctuations can be observed from capillary batch to

SUBMITTER: Roca S 

PROVIDER: S-EPMC12449748 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature