Randomized Controlled Preclinical Trial of a Benzodiazepine-Dihydropyridine Hybrid Molecule in Rodent Stroke.

ABSTRACT:

Background

The 3-ethoxycarbonyl-2-methyl-4-(2-nitrophenyl)-4,11-dihydro-1H-pyrido[2,3-b][1,5]benzodiazepine (JM-20) molecule is a novel multifunctional molecule with potent neuroprotective effects in rat focal cerebral ischemia. To confirm previous results obtained in single laboratories with small sample sizes, and to provide a robust preclinical evidence base for potential clinical development in stroke, we performed a 2-center preclinical trial with sufficiently large group sizes to detect relevant effects, minimizing biases in experimental design as much as possible (randomization, blinding, predefined in- and exclusion criteria) and increasing external and construct validities.

Methods

Experimental focal cerebral ischemia was induced by different surgeons in 2 different

SUBMITTER: Ramirez-Sanchez J 

PROVIDER: S-EPMC12449987 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature