Two-photon lithography-fabricated deterministic lateral displacement microfluidic system for efficient minicell purification in cancer therapy.

ABSTRACT: Chromosome-less minicells, derived from aberrant polar division events of bacterial cells, have emerged as promising nanocarriers for targeted cancer drug delivery due to their unique characteristics. A major challenge in their purification process lies in effectively isolating such spherical minicells (< 1 μm) from their rod-shaped parental cells (1-10 μm). This study investigates the use of Deterministic Lateral Displacement (DLD) microfluidic systems for minicell purification, leveraging Two-Photon Lithography (TPL) for the rapid prototyping of high-resolution designs optimized for this purpose. Under laminar flow conditions, we investigated key DLD design parameters including symmetric and asymmetric post gaps, outlet widths, dual post arrays, fluidic-resistance-optimized design. To en

SUBMITTER: Sharavu SH 

PROVIDER: S-EPMC12450229 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature