Creation and validation of a proteome-wide yeast library for protein detection and analysis.

ABSTRACT: A significant challenge in cell biology is to uncover the function of uncharacterized proteins. Surprisingly, a quarter of the proteome is still poorly understood even in the most well-studied model organisms. Systematic methodologies, including the use of tagged protein collections, have emerged as a powerful approach to address this gap. Despite the availability of proteome-wide collections featuring various fused proteins, the impact of different tags on protein function highlights the need for diversifying the tags used for functional genomic studies. To rise to this challenge, we created a proteome-wide collection of yeast strains in which proteins are N-terminally tagged with the broadly utilized and compact hemagglutinin (HA) epitope. We showcase the potential uses of our library fo

SUBMITTER: Baruch D 

PROVIDER: S-EPMC12450460 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature