Effect of the autoimmune-associated genetic variant <i>PTPN22</i> R620W on neutrophil activation and function in patients with insulin-dependent diabetes mellitus.

ABSTRACT:

Introduction

Recent evidence highlights neutrophils' role in initiating/sustaining aberrant immune responses in type 1 diabetes (T1D). The PTPN22 C1858T variant, a risk factor for several autoimmune conditions including T1D, affects T/B-cell receptor signaling. This study investigates its contribution to the altered neutrophil activation and function in T1D.

Materials and methods

Neutrophils were isolated from peripheral blood mononuclear cells (PBMCs) of wild-type (WT) C1858C, heterozygous (HET) C1858T T1D patients, and healthy controls (HD). Reactive oxygen species (ROS) levels were assessed using a fluorogenic probe by Fluorescence- Activated Cell Sorting (FACS) in tumor necrosis factor-alpha (TNF-α) primed, unprimed-N-formylmethionyl-leucyl-phenylalanine (f

SUBMITTER: Belcastro E 

PROVIDER: S-EPMC12450659 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature