Bioinspired reconfigurable cloverleaf DNA origami as a versatile platform for visual molecular detection.

ABSTRACT: DNA origami technology has revolutionized nanofabrication by enabling the evolution from static nanostructures to programmable dynamic systems. Herein, we present a bioinspired cloverleaf DNA origami system enabling structural reconfiguration, reversible molecular detection, and programmable higher-order assembly. Two basic cloverleaf related DNA origami structures were designed: a flexible four-leaf structure (Leaf4) and a stable four-leaf clover (Lucky4) stabilized by central linker staples, as confirmed by atomic force microscopy (AFM) and simulations (tacoxDNA, CanDo). Leaf4 transitions to a closed doughnut topology via apex strand replacement, mimicking water lily closure dynamics. Lucky4 serves as a sensing platform: its blades are functionalized with split capture strands, en

SUBMITTER: Sun S 

PROVIDER: S-EPMC12451526 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature