A customized affordable multiplexed immunofluorescence method visualizes early changes in the mouse brain microenvironment upon laser cytoreduction.

ABSTRACT:

Introduction

Multiplex immunofluorescence (mIF) utilizes distinct fluorophore-conjugated antibodies to enable the simultaneous visualization and quantification of multiple protein targets within a single tissue section. mIF allows high-resolution spatial mapping of cellular phenotypes within the native tissue microenvironment (TME). mIF facilitates the comprehensive analysis of complex biological systems, such as brain tumors, immune cell infiltration, and tissue heterogeneity. Laser interstitial thermal therapy (LITT) is a minimally invasive, hyperthermia-based laser cytoreductive method for the treatment of surgically inaccessible brain tumors, treatment-resistant epilepsy, and radiation necrosis. Laser-induced heat causes tissue damage, vascular leakage, and the appearance of he

SUBMITTER: Shanmugam Anandhan S 

PROVIDER: S-EPMC12452037 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature