HPL-conditioned MSC-derived extracellular vesicles exhibit enhanced anticancer effects on MCF7 and A549 cells.

ABSTRACT:

Introduction

Mesenchymal stem cells (MSCs) have therapeutic potential due to their differentiation and immune-regulatory capabilities, mainly through the secretion of extracellular vesicles (EVs) which can exhibit pro- and anti-tumor effects.

Aims

This study aimed to investigate the effects of EVs derived from Whorton's jelly derived MSCs (WJMSCs) cultured in fetal bovine serum (FBS) or human platelet lysate (hPL), as xeno-free alternative, on breast (MCF7) and lung (A549) cancer cells.

Methods

EVs were isolated from WJMSCs cultured in either FBS or hPL and then applied to cancer cells. Following characterization of EVs, the effects on cancer cell proliferation, apoptosis, senescence, and migration were also assessed.

Results

Both EV types reduced proliferation

SUBMITTER: Ababneh NA 

PROVIDER: S-EPMC12452434 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature